Journal: Oncology letters

Article Title: miR-221 regulates proliferation and apoptosis of ovarian cancer cells by targeting BMF.

doi: 10.3892/ol.2018.9446

Figure Lengend Snippet: Figure 5. Transfection of anti‑miR‑221 or pIRES2‑BMF significantly promoted the apoptosis of SKOV3 cells. (A) Detection of apoptosis by flow cytometry. (B) Statistical comparison of apoptosis in each transfected group. (C) Spectrophotometry detected the activity of caspase‑3. *P<0.05, comparison between two groups. miR‑221, microRNA‑221; NC, negative control; BMF, B‑cell lymphoma 2 modifying factor; si, small interfering; pIRES2, phosphorylated internal ribosome entry site 2.

Article Snippet: Cells were re-suspended in 100 μl binding buffer (part of the apoptosis detection kit), then cells were incubated with a cell apoptosis detection kit [10 μl (15 M) Annexin V dye (FITC) and 5 μl (15 M) PI dye; Beyotime Institute of Biotechnology, Haimen, China] in the dark for 15 min at 37 ̊C.

Techniques: Transfection, Flow Cytometry, Comparison, Spectrophotometry, Activity Assay, Negative Control